Acta Universitatis Medicinalis Anhui

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Anhui Provincial Education Department

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Anhui Medical University

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Weng Jianping

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Editorial Board of Acta Universitatis Medicinalis Anhui Meishan Road , Hefei 230032

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ISSN 1000 - 1492

CN 34 - 1065/R

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Analysis of the early efficacy of anterior cervical zero-profile interbody fusion and traditional titanium plate combined interbody fusion in the treatment of three-segment cervical spondylosis

Shan Tao, Wang Fei, Li Chun, Dong Zhou, Ni Zhihao, Zheng Ke

DOI: 专辑：医药卫生科技

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Abstract:
Objective To evaluate the comparative clinical outcomes of zero-profile interbody fusion (Zero-P) and traditional anterior cervical discectomy and fusion with titanium plate (ACDF- plate) for three-level cervical spondylosis, providing a reference to optimize surgical strategies for three-level cervical spondylosis. Methods In this retrospective comparative study, 113 patients were included and assigned to either the Zero-P group (n=51) or the ACDF-plate group (n=62) according to their surgical approaches. Clinical outcomes were evaluated and compared between the two groups using operative time, intraoperative blood loss, length of hospital stay, visual analog scale (VAS) scores, cervical range of motion (ROM), neck disability index (NDI), Cervical Disability Index (CDI), Japanese orthopaedic association (JOA) scores, and postoperative complication rates. Results Compared with the ACDF-plate group, the Zero-P group had a shorter operative time and less intraoperative blood loss (P<0.05); the neck pain VAS score was lower on the 1st day and 1st week after surgery (P<0.05); the cervical flexion range of motion was higher at 1 month after surgery (P<0.05); the JOA score was higher at 3 months after surgery, while the NDI and CDI scores were lower (P<0.05); the total incidence of complications (11.76%) was significantly lower than that in the plate fixation group (35.48%), and the incidence of neck foreign body sensation/stiffness was lower (P<0.05). Conclusion Zero-P not only demonstrates safety and efficacy comparable to the ACDF-plate, but also highlights the significant value of the minimally invasive concept in facilitating early recovery. Both procedures have their respective emphasis on biomechanical maintenance and minimally invasive benefits, providing a clear evidence-based reference for individualized and precise surgical decision-making in three-level cervical spondylosis.

Wall-broken ganoderma lucidum spore powder protects against DEN/CCl4-induced hepatocellular carcinoma in mice by modulating the Nrf2 pathway

Jin Hai, Wan Shuo, Liu Qian, Zhao Shiyu, Li Jing, Zhu Jiaxing, An Jiaxing, Jin Feng

DOI: 专辑：医药卫生科技

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Abstract:
Objectivec To investigate the therapeutic effect of wall-broken Ganoderma lucidum spore powder (GLS) on a mouse model of hepatocellular carcinoma and to explore its underlying mechanism. Methods Fifty 14-day-old C57BL/6J mice were divided into a control group, a model group and low, medium and high-dose GLS groups(0.25, 0.5, 1.0 g/kg ), with 10 mice in each group. A DEN/CCL4 mouse hepatocellular carcinoma model was established, and GLS was administered by gavage starting from the 8th week, once a day for 14 weeks. At the end of the final administration, the number of liver nodules was observed , and the serum alpha-fetoprotein (AFP) was detected using enzyme-linked immunosorbent assay. The content of catalase (CAT) in serum and liver tissue was determined by ammonium molybdate method. The content of reduced glutathione (GSH) in serum and liver tissue was measured by microplate assay. The content of glutathione peroxidase (GSH-PX) in serum and liver tissue was detected by colorimetric method. Immunohistochemistry (IHC-P) was employed to detect the protein expression of proliferating cell nuclear antigen (PCNA). R eal-time fluorescence quantitative polymerase chain reaction was utilized to detect the relative expression of nuclear factor E2-related factor 2 (Nrf2), Kelch-like epichlorohydrin-associated protein 1 (Keap1),NAD(P)H quinone oxidoreductase 1 (Nqo1), heme oxygenase-1 ( Ho-1 ) and glutamate cysteine ligase catalytic subunit (Gclc) mRNA. Results Compared with the control group, the content of serum AFP were significantly higher in the model group (P<0.01), while the contents of CAT, GSH and GSH-PX in liver and serum were significantly lower in the model group (P<0.01). The relative expression of Keap1 and Nqo1 mRNA was significantly higher (P<0.01), the relative expression of Nrf2, Ho-1, Gclc mRNA relative expression was significantly lower (P<0.01), and the positive expression rate of PCNA was significantly higher (P<0.01). Compared with the model group, the total number of liver nodules and content of serum AFP in the administered group were significantly lower (P<0.01), and the contents of CAT, GSH, and GSH-PX in the liver and serum of mice significantly increased (P<0.05 or P<0.01). The expression of Keap1 and Nqo1 mRNA significantly decreased (P<0.05). The expression of Nrf2, Ho-1 and Gclc mRNA significantly increased (P<0.05), and positive expression rate of PCNA significantly decreased (P<0.01). Conclusion GLS has a significant protective effect on DEN/CCL4 -induced mouse hepatocellular carcinoma model, and the mechanism of action is related to the regulation of oxidative stress in mouse liver.

4-Phenylbutyrate ameliorates lipopolysaccharide-induced bile acid metabolism disorder in maternal mice

Liang Xinru ,Sun Ying, Bao Shuangrui , Zhan Tong ，Wu Hongyan ，Tao Wenkang， Zhang Lun，Wang Jianqing ，Zhang Cheng

DOI: 专辑：医药卫生科技

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Construction of a COPD risk prediction model based on machine learning and the COPD-SQ questionnaire

Chen Lin, Zhao Luna, Zhou Yue, Wang Panpan, Li Jingkun,Zhang Wenwen,Zhang Xinxin ,Wu Chao, Liu Dong

DOI: 专辑：医药卫生科技

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Abstract:
Objective This study aims to construct and evaluate various machine learning models for predicting the risk of chronic obstructive pulmonary disease (COPD) in individuals, thereby providing data support for early screening and intervention.Methods A total of 823 subjects were selected for this study, comprising l42 individuals in the high-risk group for COPD and 68l individuals in the low-risk group. Data collected included demographic characteristics, smoking history, symptoms (such as cough and shortness of breath), and scores from the Chronic obstructive pulmonary disease screening questionnaire. Four machine learning algorithms—Logistic Regression, Random Forest, Support Vector Machine, and XGBoost—were utilized to construct risk prediction models. The performance of these models was assessed using 5-fold cross-validation, with evaluation metrics including accuracy, precision, recall, Fl-score, area under the receiver operating characteristic curve (AUC), and average precision (AP). Furthermore, a feature importance analysis was performed.Results The Logistic Regression model exhibited superior performance, achieving an AUC of 0.982 and an AP of 0.939. This was closely followed by the Random Forest model, which recorded an AUC of 0.975 and an AP of 0.890. Feature importance analysis revealed that smoking history, symptoms of shortness of breath, and body weight were significant predictors. All models demonstrated robust performance in identifying low-risk populations, with precision values exceeding 0.93; however, variations were observed in their efficacy in identifying high-risk populations.Conclusion Machine learning models have proven effective in identifying individuals at high risk for COPD. Among these, the logistic regression model exhibits the best overall performance, efficiently identifying high-risk populations and serving as a valuable clinical auxiliary screening tool. Various models, each with distinct performance characteristics, are suited to different clinical screening scenarios, thereby offering targeted decision-making support for the establishment of a hierarchical and intelligent COPD screening pathway.

Semaphorin 3A promotes pathological cardiac hypertrophy: an experimental study

Wu Lifei, Zhang Jiaojiao, Zhang Xing, Li Donghang, Wang Hongbo, Cao Jimin

DOI: 专辑：医药卫生科技

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Abstract:
Objective To investigate the effect of Semaphorin 3A (Sema3A) on the progression of pathological cardiac hypertrophy. Methods Wild-type C57BL 6J mice, Sema3A global overexpressing (Sema3A/Cre-ERTM) mice, and their littermate controls (Cre-ERTM) were used, with 6 mice per group. A pressure-overload induced cardiac hypertrophy model was established via transverse aortic constriction (TAC), with a sham-operated group (Sham) as the control. Cardiac hypertrophy was assessed by gross morphology, heart weight/body weight ratio (HW/BW), heart weight/tibia length ratio (HW/HL), and heart weight/lung weight ratio (HW/PW). Cardiac function was evaluated by measuring ejection fraction (EF) and fractional shortening (FS) via echocardiography. In heart tissues, the mRNA level of atrial natriuretic peptide (ANP) and Sema3A were detected by RT-qPCR, Sema3A protein level was detected by western blot, morphological structure was detected by hematoxylin and eosin (HE) staining, level of fibrosis was detected by Masson’s trichrome staining, and angiogenesis was detected by immunohistochemical detection of CD31.Forin vitroexperiments, rat H9C2 cardiomyocytes were treated with angiotensin II (Ang II) to induce hypertrophy, with PBS-treated cells as the control. After 48 hours, the mRNA expression of ANP, B-type natriuretic peptide (BNP) and Sema3A were measured by RT-qPCR, and Sema3A protein level was assessed by western blot.Results At the animal level, compared with the sham group, mice subjected to TAC showed significantly increased mRNA levels of ANP in cardiac tissue, as well as markedly elevated mRNA and protein levels of Sema3A (P<0.05). Compared with the Cre-ERTM + TAC group, the Sema3A/Cre-ERTM + TAC group exhibited more pronounced cardiac hypertrophy, greater ventricular wall thickening, higher HW/BW, HW/HL, and HW/PW ratios, more significant reductions in EF and FS, more severe cardiac fibrosis, and a significant decrease in cardiac CD31 expression levels (P<0.05).At the cellular level, compared with the control group, H9C2 cells treated with Ang II displayed significantly increased mRNA levels ofANP and BNP, along with markedly elevated mRNA and protein levels of Sema3A (P<0.05).ConclusionSema3A promotes the progression of pressure overload-induced cardiac hypertrophy by inhibiting angiogenesis.

Clinical characteristics and risk factors of sepsis-associated acute liver injury

Xu Lunbing, Ji Qiang, Shao Min

DOI: 专辑：医药卫生科技

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Observation on the clinical efficacy of different administration methods of G-CSF in the treatment of patients with unexplained repeated implantation failure

Zhang Yongjing, Wang Minjie, Wang Yifei， Wang Jianye, Wang Chao, Xu Yuping, Wang Tianjuan, Hao Yan, Xing Qiong

DOI: 专辑：医药卫生科技

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Abstract:
Objective To focused on patients with unexplained repeated implantation failure (URIF) and compare the clinical efficacy of different administration routes of granulocyte colony- stimulating factor (G-CSF) in patients undergoing frozen-thawed embryo transfer cycles.Methods A retrospective case-control study was conducted. A total of 187 patients with unexplained recurrent implantation failure (URIF) who received medical treatment were enrolled in this study. According to whether G-CSF was used on the day of endometrial transformation and its administration route in frozen-thawed embryo transfer cycles, the patients were divided into three groups: control group (without G-CSF, n = 71), subcutaneous injection group (subcutaneous G- CSF, n = 72), and intrauterine infusion group (intrauterine G-CSF infusion, n = 44). The pregnancy outcomes among the three groups were compared, and Logistic regression analysis was performed. Results There were significant differences in biochemical pregnancy rates among the three groups (P < 0.05), indicating statistical significance in the overall comparison. In terms of numerical trend, the biochemical pregnancy rate was in the order: subcutaneous injection group > intrauterine infusion group > control group. Further pairwise comparison showed that only the difference between the subcutaneous injection group and the control group was statistically significant (P < 0.017), while no significant differences were found between the other groups (P > 0.017). The overall comparison of early miscarriage rates among the three groups also showed a significant difference (P < 0.05), indicating a statistically significant difference in the distribution among the groups. The numerical trend was: intrauterine perfusion group > subcutaneous injection group > control group. Pairwise comparison results showed that only the difference between the intrauterine perfusion group and the control group was statistically significant (P < 0.017), while there was no statistically significant difference between any other two groups (P > 0.017).Multivariate Logistic regression analysis showed that endometrial thickness on the day of transformation, number of transferred embryos, and subcutaneous G-CSF injection were independent factors associated with an increased biochemical pregnancy rate, where as age was an independent factor for a decreased biochemical pregnancy rate. For clinical pregnancy outcome, endometrial thickness on the day of transformation, number of transferred embryos, and subcutaneous G-CSF injection were also independent factors for an increased clinical pregnancy rate, while BMI was an independent factor for a decreased clinical pregnancy rate. Conclusion Subcutaneous injection of G-CSF only improved the biochemical pregnancy rate in URIF patients. Multivariate Logistic regression analysis showed that subcutaneous G-CSF injection was a favorable factor for clinical pregnancy rate in URIF patients; however, no significant improvement in clinical pregnancy rate was observed among the three groups. Both the comparison of pregnancy outcomes and the results of multivariate logistic regression analysis indicates that intrauterine perfusion of G-CSF has no significant therapeutic effect in the treatment of patients with URIF.

Construction and validation of IL-13Rα CAR-targeted THP-1 macrophages based on OX40Lintracellular domain

Xue Hui, Xue Zimeng, Chen Weile, Qiu Jiaqi, Zhu Mengjuan, Chen Yizhao, Tu Jiajie

DOI: 专辑：医药卫生科技

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Abstract:
Objective To construct a chimeric antigen receptor macrophage (CAR-M) targeting interleukin-13 receptor α (IL-13Rα) and intracellular domain integration, which can double activate the signal adaptor protein OX40 L of macrophages and T cells. Methods The CAR molecule targeting IL- 13Rα (IL-13Rα-OX40L-CAR) with OX40L as the intracellular signal domain was designed and synthesized, and it was cloned into the adenovirus expression vector. After THP-1 cells were induced to differentiate into macrophages (THP-1-M) by phorbol ester, the cells were infected with recombinant adenovirus to construct THP-1 macrophages stably expressing green fluorescent protein (GFP) labeled anti-IL- 13RαCAR. The expression efficiency of CAR molecules was monitored by flow cytometry. The empty vector group was used as the negative control, the CD3ζ group was used as the positive control, and the CD3ζ-OX40L group was used as the experimental group. The phagocytic function of macrophages in each group on IL- 13Rα high expression glioma cells (U251) and low expression glioma cells (T98G) was detected by in vitro co-culture experiments. Results CAR adenovirus was efficiently transfected into THP-1-M to obtain anti-IL- 13RαCAR-M. After co-cultured with glioma cells with different expression levels of IL- 13Rα, the results of flow cytometry showed that there was no significant difference among the three groups for T98G cells with low expression of IL- 13Rα . After co-culture with U251 cells with high expression of IL- 13Rα, the CD3ζ group showed enhanced phagocytosis compared with the empty vector group (P<0.05). Compared with the CD3ζ group, the phagocytosis ability of the CD3ζ-OX40L group was further enhanced (P<0.05). Conclusion Anti-IL13Rα-OX40L-CAR-M is successfully constructed by constructing IL- 13Rα-OX40L-CAR adenovirus and transfecting PMA-induced differentiated THP-1-M. It can engulf glioma cells with high expression of IL- 13Rα by specific targeting, and shows activation of intracellular domain superior to traditional CD3ζ .

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