〔Abstract〕 Objective To establish an in vitro culture model of rat corpora cavernous smooth muscle cells(CC‑SMCs) using a modified tissue block adherence method.Methods Corpus cavernosum smooth muscle tissue was digested with collagenase type I and subsequently cultured using an adherent method. Cells were purified via differ-ential adhesion and identified through immunofluorescence and Western blotting.Results CCSMCs began to emerge from the tissue block after 3 days, increased significantly by day 7, and converged by day 12. Postpassage, CCSMCs exhibited strong proliferation and a “peak-to-valley” phenomenon. After purification, the cells tested positive for α-smooth muscle actin(α-SMA), confirming the successful establishment of the in vitro culture model.Conclusion The modified tissue block adherence method is a cost-effective and efficient way to obtain high-purity CCSMCs.