Fund programs: National Natural Science Foundation Project(Nos.82060518,U1503125);Special Program for Scientific and Technological Development of the Xinjiang Production and Construction Corps(No.2019BC007);Shihezi University Self-Funded Support Project(No.ZZZC202134)
Authors：Wang Le; Li Weixin; Dong Yangliu; Zhao Xian; Zhu Xinli; Zhang Xuechen; Zhe Xiangyi; Pan Zemin
Keywords：cervical cancer; bromodomain protein 4; human papillomavirus16; viral load; MZ1;
DOI：10.19405/j.cnki.issn1000-1492.2025.06.015
〔Abstract〕 Objective To explore the relationship between the replication-associated bromodomain protein 4 ( BRD4)and human papillomavirus (HPV) 16(HPV16) viral load in cervical squamous cell carcinoma and CIN Ⅰ tissues , confirm the effects of BRD4 degradation agent MZ1 on viral load . Methods Thirty HPV16-positive cervi- cal cancer specimens and 30 non-cervical cancer specimens were collected , and the viral load of the samples was detected by real-time fluorescence quantitative PCR , and the expression of BRD4 was analyzed by immunohisto- chemistry and Western blot. Results The viral load was higher in the samples of cervical cancer group than in the samples of non-cancer group , the difference is statistically significant ( P < 0. 01) . Immunohistochemistry results showed that the expression of BRD4 were significantly higher in cervical cancer specimens than in noncancerous specimens , the difference is statistically significant (P < 0. 05) . BRD4 expression was significantly and positively correlated with high viral loads , the difference is statistically significant (P < 0. 001) . the BRD4 degradation agent MZ1 significantly reduced the viral load , the difference is statistically significant (P < 0. 01) . Conclusion BRD4 may be involved in the replication of HPV16 virus , and BRD4 degradation agent MZ1 can inhibit the replication of HPV16 virus .