〔Abstract〕 Objective To evaluate the surface properties, mechanical properties, biocompatibility, and ablity to inhibit protein and bacterial of the biomimetic superhydrophobic dental restorative resin material (BSDRM).Methods Nano ZrO2 was modified with fluorosilane (FAS) to obtain FAS-ZrO2, which, together with nano SiO2, was incorporated into a resin matrix composed of bisphenol A-glycerolate dimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), perfluoroalkyl acrylate (FMA), and a photoinitiator system to fabricate BSDRM. The chemical structure and surface morphology of the material were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). Wettability parameters, including water contact angle, sliding angle and surface energy, were measured. Mechanical performance was assessed by Vickers hardness, diametral tensile strength and compressive strength tests. Biocompatibility was evaluated using CCK-8 assays and cell staining. The ability to inhibit protein adsorption and bacterial adhesion were examined by salivary mucin adsorption and Streptococcus mutans adhesion assays, respectively. Results Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the successful grafting of FAS onto the ZrO2 surface. BSDRM exhibited a micro/nanoscale hierarchical roughness, with a water contact angle of 152.97°± 1.64°, a sliding angle of 7.37°± 1.91°, a surface energy of 1.20 ± 0.26 mN/m. Mechanical testing showed that BSDRM had significantly higher hardness than the hydrophilic resin and the commercial pit-and-fissure sealant, whereas its compressive strength was not significantly different from that of the sealant (P > 0.05). CCK-8 and cell staining results demonstrated that BSDRM was non-cytotoxic to L929 cells and preserved normal cellular morphology. In the protein adsorption assay, BSDRM showed the lowest mucin adsorption among all groups (P < 0.001). In the bacterial adhesion assay, the amount of S. mutans adhering to the BSDRM surface was significantly lower than that in the control groups (P < 0.01). Conclusions BSDRM was successfully developed in this study. The material combines excellent superhydrophobicity, favourable mechanical properties, good biocompatibility, and marked ability to inhibit protein adsorption and bacterial adhesion. By suppressing plaque formation, BSDRM may offer a promising new materials-based strategy for caries prevention.