Found programs: Natural Science Foundation of Sichuan Province (No . 2022NSFSC1306)
Authors：Zhang He , Luo Xinyue , Heng Xin , Zhang Yun , Wang Songping , Deng Jun
Keywords：pulmonary infection; metagenomic second generation sequencing; mNGS; conventional detection; alveolar lavage fluid; underlying medical conditions; pathogen;
DOI：10.19405/j.cnki.issn1000-1492.2025.10.018
〔Abstract〕 Objective To investigate the value of metagene next-generation sequencing(mNGS) in the detection of pathogens in patients with pulmonary infection. Methods A retrospective analysis was performed on clinical data from 434 patients with pulmonary infections admitted over the past four years. Based on the presence of underlying comorbidities, patients were divided into underlying disease group(n=262) and non-underlying disease group(n=172). Pathogen detection was conducted using both mNGS and conventional tests. Clinical and laboratory parameters, radiographic findings, and pathogen detection results were systematically analyzed. The diagnostic performance of the two methods in identifying causative pathogens of pulmonary infections was compared. Results The positive rate of mNGS in 434 patients was higher than that of conventional tests, and the difference was statistically significant(P