〔Abstract〕 To construct a lentiviral overexpression cell line of HACE1 and to investigate its effects on the proliferation and migration of glioma cells . Methods Using the bacterial liquid containing the full-length cDNA sequence of human HACE1 as a template , the lentiviral expression plasmid pCDH-HACE1 was constructed , and the lentiviral cell line was screened . Western blot was employed to detect the expression of HACE1 in glioma cells . Meanwhile , the CCK-8 assay and the cell scratch assay were utilized to assess the impacts of HACE1 on the prolif- eration and migration of glioma cells . Results The lentiviral cell line with HACE1 overexpression was successfully established . Western blot analysis demonstrated that HACE1 could be effectively expressed in eukaryotic cells . The results of the CCK-8 assay showed that , compared with the control group , the cell proliferation in the HACE1 over- expression group was significantly enhanced at 24 hours and 48 hours after cultivation ( t values : 2. 42 and 1 . 85 re- spectively , both P < 0. 01) . The cell scratch assay revealed that , at 24 hours and 48 hours after cultivation , the Scratch areas in the HACE1 overexpression group were higher than those in the control group ( t values : 4. 46 , P < 0. 05 ; t value : 1 . 30 , P < 0. 01) . Conclusion The lentiviral cell line with HACE1 overexpression is successfully constructed . HACE1 can promote the proliferation and migration abilities of U251 glioma cells , providing a founda- tion for further research .