〔Abstract〕 Objective To explore the effects of Kruppel-like factor 7(KLF7) on the proliferation, migration and cycle of esophageal squamous cell carcinoma cell line Eca109. Methods Bioinformatics analysis was used to study the expression pattern of KLF7 in esophageal squamous cell carcinoma and its relationship with prognosis and pathological grade. The expression pattern of KLF7 in esophageal squamous cell carcinoma cells was studied by real-time PCR, Western blot and cellular immunofluorescence in vitro. The KLF7 overexpression in Eca109 cells was carried out by transfection of the plasmid of pcDNA-3.10-KLF7, and the effects of KLF7 on the proliferation and migration of ESCC cells were detected by CCK-8 assay, plate clone formation assay and Transwell assay, and the cell cycle was studied by Flow cytometric analysis. Results KLF7 was expressed in the tissues and cells of esophageal cancer, and its expression was significantly associated with the pathological grade of esophageal cancer patients(P<0.05), however, there was no significant associaition of KLF7 expression with the survival rate of patients. The expression level of KLF7 in esophageal cancer cells(Eca109 and EC9706) was significantly higher than that in normal esophageal epithelial cells(Het-1A,P<0. 05). In addition, the expression of KLF7 was higher in the cytoplasm and nucleus of Het-1A cells, while only in the nucleus of esophageal cancer cells(Eca109 and EC9706).Additionally, the overexpression of KLF7 in Eca109 cells changed the cell cycle, promoted cell proliferation and migration, and up-regulated the expression levels of CCND1 and P53(P<0. 05).Conclusion Overexpression of KLF7 might promote the proliferation and migration of esophageal cancer cells.